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Image Search Results
Journal: Proceedings of the National Academy of Sciences of the United States of America
Article Title: Insulin suppresses the production of fibroblast growth factor 23 (FGF23)
doi: 10.1073/pnas.1800160115
Figure Lengend Snippet: Transcription factor FOXO1 regulates Fgf23 expression. (A, Upper) Original Western blots of phospho-FOXO1 (pFOXO1) and GAPDH (Left) or FOXO1 and GAPDH (Right) abundance in UMR106 cells incubated with or without 10 nM insulin for 30 min. (A, Lower) Densitometric analysis (arithmetic means ± SEM, n = 7) of phospho-FOXO1 or FOXO1 protein abundance normalized to GAPDH. (B) Arithmetic means ± SEM (n = 5) of Fgf23 mRNA abundance in UMR106 cells incubated with or without the FOXO1 inhibitor AS1842856 (50 nM). (C) Arithmetic means ± SEM (n = 5) of Fgf23 mRNA abundance in UMR106 cells treated with Foxo1-specific or nonsense siRNA as a negative control (neg. siRNA). (D) Representative Western blot and densitometric analysis (arithmetic means ± SEM, n = 7) of FOXO1 protein abundance normalized to GAPDH in Foxo1-specific or nonsense siRNA-treated (neg. siRNA) cells. (E) Arithmetic means ± SEM (n = 7) of Fgf23 mRNA in UMR106 cells transfected with a vector construct encoding human FOXO1-WT or with an empty vector pEGFP-C1 as a control. (F) Representative original Western blot (Left) demonstrating FOXO1 and the GFP-FOXO1 fusion protein abundance and representative microscopy image (Right) showing the GFP signal of the fusion protein or GFP alone in UMR106 cells following transfection with human FOXO1-WT or with an empty vector pEGFP-C1. (Scale bar: 50 μm.) *P < 0.05 and ***P < 0.001 indicate significant difference from control-treated cells. arb., arbitrary.
Article Snippet: UMR106 cells cultured for 24 h without antibiotics were transfected with pEGFPC1-hFOXO1 or with the empty vector pEGFP-C1 as a negative control (6 μg of DNA) using
Techniques: Expressing, Western Blot, Incubation, Quantitative Proteomics, Negative Control, Transfection, Plasmid Preparation, Construct, Control, Microscopy